Eliminate the Dilution Step -- Here's How:

This demonstrates the typical math required for eliminating the intermediate dilution step:

Example assumes typical desired outcomes of the dilution step:             

            < 1% DMSO for homogeneous assays

            <0.5% DMSO for cell-based assays 

Please consult Thermo Fisher Scientific specialists for "doing the math" on your assay!

Traditional method: 

          Dilution step adds time, increases risk of compound precipitation, and uses materials.

PocketTip method:

Doing the Math....How do I determine the Pocket size???

    Simply, the formula is as follows: 

            V1 = M2*V2/M1            where   V1 = Pocket size (in nL)

                                                            M1 = Source compound molarity (in mM)

                                                            M2 = Desired assay compound molarity (in μM)

                                                            V2 = Total Assay Volume (in μL)

                                                where 0.25% is the goal for DMSO in the final assay volume

    Hence, for a source at 10mM, and a desired assay plate at 10μM in a 50μL assay volume ----> a Pocket of 50nL is required

    Or, for a source at 4mM, and a desired assay plate at 10μM in a 40μL assay volume ----> a Pocket of 100nL is required, and so on.

Please consult a Thermo Fisher Scientific specialist for "doing the math" on your assay!

  nAscent BioSciences is now part of Thermo Fisher Scientific.  Contact PocketTips.Info@thermofisher.com for more information.